Screening and Identification for Antagonistic Bacteria of Lonsdalea populi
-
摘要:
目的 筛选获得欧美杨细菌性溃疡病菌杨树朗斯代尔氏菌（Lonsdalea populi ）的拮抗细菌。 方法 以欧美杨细菌性溃疡病病原菌L. populi CFCC 13731为靶标菌，采用平板拮抗法筛选拮抗菌株，并通过牛津杯法测定其防效。通过观察菌株形态、生理生化实验、16S rRNA序列分析，对高效拮抗菌株进行鉴定。 结果 筛选获得10株具有拮抗作用的菌株，复筛获得1株菌株（k1）对L. populi具有较好的拮抗效果，鉴定结果表明菌株k1为多粘类芽孢杆菌（Paenibacillus polymyxa）。 结论 筛选获得的多粘类芽孢杆菌k1对L. populi具有较好的拮抗作用，在欧美杨细菌性溃疡病防治中将具有较大的应用潜力。 Abstract:Objective The aim is to screen for antagonistic bacteria of Lonsdalea populi which causes the bark canker on Populus × euramericana in China. Method The L. populi strain CFCC 13731 was used as target, and the antagonistic strains were screened by the plate antagonism method, their preventive efficacy was determined by the Oxford cup method. The efficient antagonist strains were identified through morphological, physiological and biochemical characteristics in combination with 16S rRNA sequence analysis. Result Ten bacteria with antibacterial activity against L. populi were screened. Further screening yielded strain k1 with good antimicrobial activity, which was identified as Paenibacillus polymyxa. Conclusion Screening obtained that the P. polymyxa k1 has a good antagonistic effect on L. populi, which will have a greater potential for application in the control of the bark canker on Populus × euramericana. -
图 3 基于k1的16S rRNA基因构建的系统发育树
Figure 3. Phylogenetic tree constructed based on the 16S rRNA genes of k1
表 1 生理生化鉴定结果
Table 1. The result of physiological and biochemical characteristic
生理生化特征
Physiological and biochemical characteristick1 多粘类芽孢杆菌
Paenibacillus polymyxa ATCC 842T氧化酶活性 Oxidase activity − − H2S产生 H2S production − − 吲哚产生 Indole − − 明胶酶活性 Gelatinase activity − − 柠檬酸利用 Citrate utilization − − 精氨酸水解酶活性 Arginine dihydrolase activity + + 赖氨酸脱羧酶活性 Lysine decarboxylase activity − − 脲素 Urease − − 甘露醇 Mannitol + + 阿拉伯糖 Arabinose + + 葡萄糖 Glucose + + 蔗糖 Sucrose + + 乳糖 Lactose + + 甘油 Glycerol + + 肌醇 Inositol − − 蜜二糖 Melibiose + + 龙胆二糖 Gentiobiose − + 山梨醇 D-Sorbitol − − 苦杏仁苷 Amygdalin + + 松二糖 Turanose + + 
下载: 导出CSV
表 2 初筛获得具拮抗作用菌株
Table 2. Antagonistic strains obtained by screening
菌株
Strains所属种
Species16S rRNA序列同源性
16S rRNA sequence similarity/%来源
Isolationk1 Paenibacillus polymyxa 99.43 根际土 k2 Bacillus tequilensis 99.26 根际土 k3 Bacillus velezensis 99.93 根际土 CFCC 10524 Bacillus siamensis 99.85 山茶叶片 CFCC 1714 Bacillus amyloliquefaciens 99.56 根际土 CFCC 2826 Bacillus atrophaeus 99.92 植物根面 CFCC 10015 Bacillus velezensis 100 土壤 G-3-k Bacillus velezensis 99.93 根际土 Y-1-k Bacillus velezensis 100 根际土 G-2-3 Bacillus siamensis 99.72 根际土
下载: 导出CSV
-
[1] 陈雪丽, 王光华, 金剑, 等, 2008. 多粘类芽孢杆菌 BRF-1 和枯草芽孢杆菌 BRF-2 对黄瓜和番茄枯萎病的防治效果[J]. 中国生态农业学报, 16(2): 446-450. [2] 郭利民, 常聚普, 贺伟, 等, 2012. 欧美杨溃疡病田间发生规律研究初报[J]. 林业实用技术, (10): 38-40. doi: 10.13456/j.cnki.lykt.2012.10.011 [3] 郭利民, 张鸣放, 王洪轩, 2008. 濮阳市发现杨树新病害[J]. 河南林业科技, (3): 36-36. doi: 10.3969/j.issn.1003-2630.2008.03.019 [4] 贺伟, 任飞娟, 郭利民, 等, 2009. 欧美杨溃疡病的病原鉴定[J]. 林业科学, 45(6): 104-108. doi: 10.3321/j.issn:1001-7488.2009.06.018 [5] 金莉萍, 2016. 多粘类芽孢杆菌生防促生机制研究及发酵条件的优化[D]. 杭州: 浙江农林大学. [6] 任飞娟, 2011. 欧美杨溃疡病病原鉴定[D]. 北京: 北京林业大学. [7] 徐方媛, 贺伟, 常聚普, 等, 2013. 欧美杨溃疡病防治的初步研究[J]. 中国农学通报, 29(16): 12-17. doi: 10.11924/j.issn.1000-6850.2013-0174 [8] 于成明, 伦莹莹, 刘会香, 等, 2014. 杨树细菌性溃疡病菌的室内药剂活性筛选[J]. 农药科学与管理, 35(7): 56-58. doi: 10.3969/j.issn.1002-5480.2014.07.015 [9] 王海明, 牛迎福, 王永立, 等, 2015. 欧美杨细菌性溃疡病发生与危害林间观察[J]. 中国森林病虫, 34(2): 19-22. doi: 10.3969/j.issn.1671-0886.2015.02.005 [10] Abelleira A, Moura L, Aguín O, et al, 2019. First report of Lonsdalea populi causing bark canker disease on poplar in Portugal[J]. Plant Disease, 103(8): 2121. doi: 10.1094/PDIS-12-18-2230-PDN [11] Berruete I M, Cambra M A, Collados R, et al, 2016. First report of bark canker disease of poplar caused by Lonsdalea quercina subp. populi in Spain[J]. Plant Disease, 100(10): 2159-2159. doi: 10.1094/PDIS-03-16-0405-PDN [12] Biosca E G, González R, López-López M J, et al, 2003. Isolation and characterization of Brenneria quercina, causal agent for bark canker and drippy nut of Quercus spp. in Spain[J]. Phytopathology, 93(4): 485. doi: 10.1094/PHYTO.2003.93.4.485 [13] Brady C L, Cleenwerck I, Denman S, et al, 2012. Proposal to reclassify Brenneria quercina (Hildebrand and Schroth 1967) Hauben et al. 1999 into a new genus, Lonsdalea gen. nov., as Lonsdalea quercina comb. nov., descriptions of Lonsdalea quercina subsp. quercina comb. nov., Lonsdalea quercina subsp. iberica subsp. nov. and Lonsdalea quercina subsp. britannica subsp. nov., emendation of the description of the genus Brenneria, reclassification of Dickeya dieffenbachiae as Dickeya dadantii subsp. dieffenbachiae comb. nov., and emendation of the description of Dickeya dadantii[J]. International Journal of Systematic and Evolutionary Microbiology, 62(Pt_7): 1592-1602. doi: 10.1099/ijs.0.035055-0 [14] Kumar S, Stecher G, Li M, et al, 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms[J]. Molecular Biology and Evolution, 35(6): 1547. doi: 10.1093/molbev/msy096 [15] Lal S, Tabacchioni S, 2009. Ecology and biotechnological potential of Paenibacillus polymyxa: a minireview[J]. Indian Journal of Microbiology, 49(1): 2-10. doi: 10.1007/s12088-009-0008-y [16] Li Y, He W, Ren F J, et al, 2014. A canker disease of Populus× euramericana in China caused by Lonsdalea quercina subsp. populi[J]. Plant Disease, 98(3): 368-378. doi: 10.1094/PDIS-01-13-0115-RE [17] Li Y, Xue H, Guo L M, et al, 2017. Elevation of three subspecies of Lonsdalea quercina to species level: Lonsdalea britannica sp. nov., Lonsdalea iberica sp. nov. and Lonsdalea populi sp. nov.[J]. International Journal of Systematic and Evolutionary Microbiology, 67(11): 4680-4684. doi: 10.1099/ijsem.0.002353 [18] Tóth T, Lakatos T, Koltay A, 2013. Lonsdalea quercina subsp. populi subsp. nov., isolated from bark canker of poplar trees[J]. International Journal of Systematic and Evolutionary Microbiology, 63(Pt_6): 2309-2313. doi: 10.1099/ijs.0.042911-0 -
点击查看大图
图(3) / 表(2)
计量
- 文章访问数: 91
- HTML全文浏览量: 48
- PDF下载量: 9
- 被引次数: 0
