Morphological Characteristics of <i>Erannis ankeraria</i> Nucleopolyhedrovirus and Establishment of Molecular Detection Technique

Shaobo Wang; Danjie Xie; Zehui Zhang; Yunpeng Liu; Dezhi Kong; qinghua Wang; Liangjian Qu

doi:10.12356/j.2096-8884.2024-0008

Volume 4 Issue 1

Feb. 2024

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Article Navigation > Terrestrial Ecosystem and Conservation > 2024 > 4(1): 59-65

Shaobo Wang, Danjie Xie, Zehui Zhang, Yunpeng Liu, Dezhi Kong, qinghua Wang, Liangjian Qu. Morphological Characteristics of Erannis ankeraria Nucleopolyhedrovirus and Establishment of Molecular Detection Technique[J]. Terrestrial Ecosystem and Conservation, 2024, 4(1): 59-65. doi: 10.12356/j.2096-8884.2024-0008

Citation:

Shaobo Wang, Danjie Xie, Zehui Zhang, Yunpeng Liu, Dezhi Kong, qinghua Wang, Liangjian Qu. Morphological Characteristics of Erannis ankeraria Nucleopolyhedrovirus and Establishment of Molecular Detection Technique[J]. Terrestrial Ecosystem and Conservation, 2024, 4(1): 59-65. doi: 10.12356/j.2096-8884.2024-0008

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Morphological Characteristics of Erannis ankeraria Nucleopolyhedrovirus and Establishment of Molecular Detection Technique

doi: 10.12356/j.2096-8884.2024-0008

1.
Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China
2.
Beijing Blue Fox Natural Enemy Technology Company Limited, Beijing 100089, China
3.
North Mandian Division of Saihanba Mechanized Forest Farm of Hebei Province, Weichang 068450, China

Received Date: 2024-01-30
Available Online: 2024-04-18
Publish Date: 2024-02-01

Abstract

Abstract

Objective Erannis ankeraria nucleopolyhedrovirus (EranNPV) is a significant natural enemy microbial product that effectively regulates the population and quality of E. ankeraria. The elucidation of the ultramicromorphological characteristics of EranNPV and the establishment of a sensitive and convenient PCR-based rapid detection method will help to further investigate the epidemiology and transmission mechanisms of EranNPV in forest ecosystems. This, in turn, provides technical support for the continuous control of E. ankeraria using EranNPV. Method The external morphology and internal structural features of EranNPV polyhedra were observed using scanning electron microscopy and transmission electron microscopy. A pair of PCR detection primers, EaV, was designed based on the ac54 gene of EranNPV. The specificity of EaV was examined by PCR amplification results of various insect viruses. The sensitivity of PCR amplification using EaV primers was determined by testing different concentrations of EranNPV genomic DNA and polyhedrons. Result The electron microscopy results showed that the majority of the virus polyhedra were irregular polyhedra with smooth surfaces, and a few had scattered pore depressions with an average diameter of 1.43 ± 0.20 μm. The virus particles within the polyhedra were distributed in a single bundle with an average length of 219.14 ± 17.50 nm. When EaV is used as the primer, only EranNPV can be amplified to the target band. The PCR assay using EaV primers exhibited a sensitivity of 10 fg/mL for EranNPV genomic DNA and a sensitivity of 1×10² OBs/mL for polyhedrons. Conclusion EranNPV is a typical single nucleocapsid nucleopolyhedrovirus. The PCR assay using EaV primers demonstrates high specificity and sensitivity. It is expected to be further applied to the study of the diffusion and transmission mechanism of EranNPV in forest ecosystems.
- Erannis ankeraria,
- nucleopolyhedrovirus,
- scanning electron microscope,
- transmission electron microscopegraph,
- PCR detection

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References(26)

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