Identification of Pestalotiopsis Species Based on Morphology and Molecular Phylogeny
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摘要:
目的 鉴定存于中国林业微生物菌种保藏管理中心的99株拟盘多毛孢菌株，并探究种间差异。 方法 开展联合多基因片段（ITS-tef1-tub2）分子系统学和形态学研究，并采用统计学手段对各物种的形态指标进行评估。 结果 分子系统学和形态学结果显示这些菌株为7个物种，即冷杉拟盘多毛孢（1株）、矮棕拟盘多毛孢（18株）、肯尼亚拟盘多毛孢（19株）、庐山拟盘多毛孢（38株）、南京拟盘多毛孢（3株）、葡萄牙拟盘多毛孢（13株）和桃金娘拟盘多毛孢（7株）。通过分生孢子长度、分生孢子宽度、长宽比、顶附属丝长度和底附属丝长度这5项指标中的1项或多项可有效区分此7种。 结论 研究揭示了中国林业微生物菌种保藏管理中心保存的7种拟盘多毛孢的形态学和分子系统学特征；建议重视顶附属丝长度这一形态指标在该属分类鉴定中的应用。 Abstract:Objective The aim of the present study was to identify 99 Pestalotiopsis isolates kept in China Forestry Culture Collection Center (CFCC) and to study the interspecific differences. Methods Isolates were identified by combined methods of phylogeny based on ITS-tef1-tub2 loci and morphology. Conidial indicators were further evaluated by statistical method. Results The 99 isolates were identified as P. abietis (1 isolate), P. chamaeropis (18 isolates), P. kenyana (19 isolates), P. lushanensis (38 isolates), P. nanjingensis (3 isolates), P. portugalica (13 isolates) and P. rhodomyrtus (7 isolates). These species can be validly distinguished by morphological data of conidial length and width, length width ratio, length of apical and basal appendages. Conclusion The present study revealed the species diversity of Pestalotiopsis isolates kept in CFCC, and morphological and phylogenetical characters were analyzed. The length of apical appendage is a useful character to separate species within Pestalotiopsis, and is recommended to be further used in the taxonomy study of this genus. -
Key words:
- Ascomycota /
- Sporocadaceae /
- taxonomy /
- identification
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图 1 拟盘多毛孢属的ITS-tef1-tub2三基因联合系统学分析
注：最大似然法支持率 ≥ 50%的数值标示在分枝节点。Maximum likelihood bootstrap support values ≥ 50% are given at the nodes.
Figure 1. Phylogram of Pestalotiopsis based on ITS- tef1-tub2 genes
图 9 拟盘多毛孢分生孢子各指标的冗余分析和相关性
Figure 9. Principal components analysis (PCA) and correlation of various conidial indexes
表 1 菌株信息
Table 1. Isolates and GenBank accession numbers
物种
Species寄主
Host分布
Distribution菌株
Isolate登录号 GenBank accession numbers ITS tub2 tef1 冷杉拟盘多毛孢
Pestalotiopsis abietis未知 湖北 CFCC 5528 OM746218 OM839891 OM839990 矮棕拟盘多毛孢
P. chamaeropis未知 未知 CFCC 55091 OM746219 OM839892 OM839991 麻栎 Quercus acutissima 湖南 CFCC 55154 OM746220 OM839893 OM839992 麻栎 Q. acutissima 四川 CFCC 55124 OM746221 OM839894 OM839993 月季 Rosa chinensis 甘肃 CFCC 55156 OM746222 OM839895 OM839994 麻栎 Q. acutissima 安徽 CFCC 54977 OM746223 OM839896 OM839995 槲栎 Q. aliena 安徽 CFCC 55019 OM746224 OM839897 OM839996 栓皮栎 Q. variabilis 河南 CFCC 53874 OM746225 OM839898 OM839997 麻栎 Q. acutissima 河南 CFCC 53873 OM746226 OM839899 OM839998 未知 河南 CFCC 50705 OM746227 OM839900 OM839999 茶 Camellia sinensis 陕西 CFCC 81670 OM746228 OM839901 OM840000 槲栎 Q. aliena 安徽 CFCC 55122 OM746229 OM839902 OM840001 未知 河南 CFCC 50719 OM746230 OM839903 OM840002 未知 未知 CFCC 50746 OM746231 OM839904 OM840003 未知 未知 CFCC 50736 OM746232 OM839905 OM840004 黧蒴锥 Castanopsis fissa 广东 CFCC 55023 OM746233 OM839906 OM840005 栓皮栎 Q. variabilis 安徽 CFCC 54776 OM746234 OM839907 OM840006 栓皮栎 Q. variabilis 陕西 CFCC 55338 OM746235 OM839908 OM840007 麻栎 Q. acutissima 河南 CFCC 55142 OM746236 OM839909 OM840008 肯尼亚拟盘多毛孢
P. kenyana槲栎 Q. aliena 贵州 CFCC 54962 OM746237 OM839910 OM840009 饭甑青冈 Cyclobalanopsis fleuryi 海南 CFCC 55330 OM746238 OM839911 OM840010 锐齿栎 Q. aliena var. acutiserrata 未知 CFCC 54621 OM746239 OM839912 OM840011 杉木 Cunninghamia lanceolata 安徽 CFCC 51440 OM746240 OM839913 OM840012 水竹 Phyllostachys heteroclada 安徽 CFCC 51519 OM746241 OM839914 OM840013 杉木 Cunninghamia lanceolata 福建 CFCC 5143 OM746242 OM839915 OM840014 竹叶青冈 Cyclobalanopsis neglecta 海南 CFCC 54732 OM746243 OM839916 OM840015 桉树 Eucalyptus robusta 云南 CFCC 82397 OM746244 OM839917 OM840016 红锥 Castanopsis hystrix 广东 CFCC 54742 OM746245 OM839918 OM840017 未知 湖北 CFCC 84762 OM746246 OM839919 OM840018 七叶树 Aesculus chinensis 陕西 CFCC 81667 OM746247 OM839920 OM840019 锐齿栎 Q. aliena var. acutiserrata 未知 CFCC 54618 OM746248 OM839921 OM840020 南天竹 Nandina domestica 云南 CFCC 82396 OM746249 OM839922 OM840021 水竹 Phyllostachys heteroclada 安徽 CFCC 51538 OM746250 OM839923 OM840022 桃 Prunus persica 贵州 CFCC 86596 OM746251 OM839924 OM840023 未知 未知 CFCC 57473 OM746252 OM839925 OM840024 青冈 Cyclobalanopsis glauca 广东 CFCC 54805 OM746253 OM839926 OM840025 黧蒴锥 Castanopsis fissa 海南 CFCC 55088 OM746254 OM839927 OM840026 石榴 Punica granatum 云南 CFCC 82395 OM746255 OM839928 OM840027 庐山拟盘多毛孢
P. lushanensis松属 Pinus 湖北 CFCC 83998 OM746256 OM839929 OM840028 湿地松 Pinus elliottii 四川 CFCC 5740 OM746257 OM839930 OM840029 未知 未知 CFCC 50588 OM746258 OM839931 OM840030 华山松 Pinus armandii 四川 CFCC 5725 OM746259 OM839932 OM840031 马尾松 Pinus massoniana 四川 CFCC 5739 OM746260 OM839933 OM840032 未知 河南 CFCC 50604 OM746261 OM839934 OM840033 湿地松 Pinus elliottii 四川 CFCC 5729 OM746262 OM839935 OM840034 山茶 Camellia japonica 四川 CFCC 5750 OM746263 OM839936 OM840035 华山松 Pinus armandii 四川 CFCC 5724 OM746264 OM839937 OM840036 未知 未知 CFCC 50742 OM746265 OM839938 OM840037 油松 Pinus tabuliformis 四川 CFCC 5720 OM746266 OM839939 OM840038 马尾松 Pinus massoniana 四川 CFCC 5708 OM746267 OM839940 OM840039 华山松 Pinus armandii 四川 CFCC 5714 OM746268 OM839941 OM840040 马尾松 Pinus massoniana 四川 CFCC 5727 OM746269 OM839942 OM840041 庐山拟盘多毛孢
P. lushanensis华山松 Pinus armandii 四川 CFCC 5726 OM746270 OM839943 OM840042 云南松 Pinus yunnanensis 四川 CFCC 5721 OM746271 OM839944 OM840043 马尾松 Pinus massoniana 重庆 CFCC 5746 OM746272 OM839945 OM840044 马尾松 Pinus massoniana 福建 CFCC 80850 OM746273 OM839946 OM840045 马尾松 Pinus massoniana 四川 CFCC 5712 OM746274 OM839947 OM840046 马尾松 Pinus massoniana 四川 CFCC 5731 OM746275 OM839948 OM840047 凋落物 黑龙江 CFCC 88486 OM746276 OM839949 OM840048 未知 重庆 CFCC 87025 OM746277 OM839950 OM840049 水竹 Phyllostachys heteroclada 安徽 CFCC 51480 OM746278 OM839951 OM840050 蕨科 Pteridiaceae 云南 CFCC 82398 OM746279 OM839952 OM840051 马尾松 Pinus massoniana 四川 CFCC 5711 OM746280 OM839953 OM840052 未知 重庆 CFCC 87024 OM746281 OM839954 OM840053 枹栎 Q. serrata 贵州 CFCC 54894 OM746282 OM839955 OM840054 油松 Pinus tabuliformis 四川 CFCC 5715 OM746283 OM839956 OM840055 华山松 Pinus armandii 四川 CFCC 5742 OM746284 OM839957 OM840056 湿地松 Pinus elliottii 四川 CFCC 5709 OM746285 OM839958 OM840057 水竹 Phyllostachys heteroclada 安徽 CFCC 51533 OM746286 OM839959 OM840058 土壤 福建 CFCC 80849 OM746287 OM839960 OM840059 马尾松 Pinus massoniana 四川 CFCC 5707 OM746288 OM839961 OM840060 马尾松 Pinus massoniana 四川 CFCC 5713 OM746289 OM839962 OM840061 马尾松 Pinus massoniana 四川 CFCC 5716 OM746290 OM839963 OM840062 马尾松 Pinus massoniana 四川 CFCC 5705 OM746291 OM839964 OM840063 大型真菌 云南 CFCC 5534 OM746292 OM839965 OM840064 松属 Pinus 未知 CFCC 5515 OM746293 OM839966 OM840065 南京拟盘多毛孢
P. nanjingensis腐木 未知 CFCC 52206 OM746294 OM839967 OM840066 槲栎 Q. aliena 河南 CFCC 53882 OM746295 OM839968 OM840067 未知 湖南 CFCC 5521 OM746296 OM839969 OM840068 葡萄牙拟盘多毛孢
P. portugalica山茶 Camellia japonica 四川 CFCC 5753 OM746297 OM839970 OM840069 山茶 Camellia japonica 四川 CFCC 5759 OM746298 OM839971 OM840070 山茶 Camellia japonica 四川 CFCC 5766 OM746299 OM839972 OM840071 山茶 Camellia japonica 四川 CFCC 5754 OM746300 OM839973 OM840072 山茶 Camellia japonica 四川 CFCC 5764 OM746301 OM839974 OM840073 山茶 Camellia japonica 四川 CFCC 5760 OM746302 OM839975 OM840074 山茶 Camellia japonica 四川 CFCC 5762 OM746303 OM839976 OM840075 山茶 Camellia japonica 四川 CFCC 5765 OM746304 OM839977 OM840076 山茶 Camellia japonica 四川 CFCC 5747 OM746305 OM839978 OM840077 山茶 Camellia japonica 四川 CFCC 5751 OM746306 OM839979 OM840078 葡萄牙拟盘多毛孢
P. portugalica未知 未知 CFCC 50751 OM746307 OM839980 OM840079 山茶 Camellia japonica 四川 CFCC 5748 OM746308 OM839981 OM840080 未知 重庆 CFCC 87022 OM746309 OM839982 OM840081 桃金娘拟盘多毛孢
P. rhodomyrtus槲栎 Q. aliena 陕西 CFCC 54733 OM746310 OM839983 OM840082 窄叶青冈 Cyclobalanopsis augustinii 贵州 CFCC 55052 OM746311 OM839984 OM840083 麻栎 Q. acutissima 湖南 CFCC 55155 OM746312 OM839985 OM840084 未知 未知 CFCC 52326 OM746313 OM839986 OM840085 未知 安徽 CFCC 87869 OM746314 OM839987 OM840086 未知 重庆 CFCC 5529 OM746315 OM839988 OM840087 水竹 Phyllostachys heteroclada 安徽 CFCC 51475 OM746316 OM839989 OM840088 
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表 2 系统发育学中用到的菌株序列信息
Table 2. Isolates and GenBank accession numbers used in the phylogeny
物种
Species菌株
Isolate登录号 GenBank accession numbers ITS tub2 tef1 蔷薇生新拟盘多毛孢 Neopestalotiopsis rosicola CFCC 51992* KY885239 KY885245 KY885243 冷杉拟盘多毛孢 Pestalotiopsis abietis CFCC 53011* MK397013 MK622280 MK622277 冷杉拟盘多毛孢 P. abietis CFCC 53012 MK397014 MK622281 MK622278 暗褐拟盘多毛孢 P. adusta ICMP 6088* JX399006 JX399037 JX399070 暗褐拟盘多毛孢 P. adusta MFLUCC 10-146 JX399007 JX399038 JX399071 梯田拟盘多毛孢 P. aggestorum LC6301* KX895015 KX895348 KX895234 梯田拟盘多毛孢 P. aggestorum LC8186 KY464140 KY464160 KY464150 漆树科拟盘多毛孢 P. anacardiacearum IFRDCC 2397* KC247154 KC247155 KC247156 油杉寄生拟盘多毛孢 P. arceuthobii CBS 434.65* KM199341 KM199427 KM199516 桃榔拟盘多毛孢 P. arenga CBS 331.92* KM199340 KM199426 KM199515 澳新拟盘多毛孢 P. australasiae CBS 114126* KM199297 KM199409 KM199499 澳新拟盘多毛孢 P. australasiae CBS 114141 KM199298 KM199410 KM199501 澳大利亚拟盘多毛孢 P. australis CBS 111503 KM199331 KM199382 KM199557 澳大利亚拟盘多毛孢 P. australis CBS 114193* KM199332 KM199383 KM199475 双毛拟盘多毛孢 P. biciliata CBS 124463* KM199308 KM199399 KM199505 双毛拟盘多毛孢 P. biciliata CBS 236.38 KM199309 KM199401 KM199506 分支拟盘多毛孢 P. brachiata LC2988* KX894933 KX895265 KX895150 分支拟盘多毛孢 P. brachiata LC8188 KY464142 KY464162 KY464152 分支拟盘多毛孢 P. brachiata LC8189 KY464143 KY464163 KY464153 芸薹拟盘多毛孢 P. brassicae CBS 170.26* KM199379 NA KM199558 茶拟盘多毛孢拟盘多毛孢 P. camelliae MFLUCC 12-0277* JX399010 JX399041 JX399074 油茶拟盘多毛孢 P. camelliae-oleiferae CSUFTCC08* OK493593 OK562368 OK507963 油茶拟盘多毛孢 P. camelliae-oleiferae CSUFTCC09 OK493594 OK562369 OK507964 矮棕拟盘多毛孢 P. chamaeropis CBS 186.71* KM199326 KM199391 KM199473 矮棕拟盘多毛孢 P. chamaeropis LC3619 KX894991 KX895322 KX895208 棒孢拟盘多毛孢 P. clavata MFLUCC 12-0268* JX398990 JX399025 JX399056 哥伦比亚拟盘多毛孢 P. colombiensis CBS 118553* KM199307 KM199421 KM199488 毛地黄拟盘多毛孢 P. digitalis MFLU 14-0208* KP781879 KP781883 NA 独立拟盘多毛孢 P. dilucida LC3232 KX894961 KX895293 KX895178 独立拟盘多毛孢 P. dilucida LC8184 KY464138 KY464158 KY464148 秤钩风拟盘多毛孢 P. diploclisiae CBS 115449 KM199314 KM199416 KM199485 秤钩风拟盘多毛孢 P. diploclisiae CBS 115587* KM199320 KM199419 KM199486 广布拟盘多毛孢 P. disseminata CBS 143904 MH554152 MH554825 MH554587 广布拟盘多毛孢 P. disseminata MEAN 1165 MT374687 MT374712 MT374699 广布拟盘多毛孢 P. disseminata MEAN 1166 MT374688 MT374713 MT374700 异毛拟盘多毛孢 P. diversiseta MFLUCC 12-0287* JX399009 JX399040 JX399073 莱东拟盘多毛孢 P. doitungensis MFLUCC 14-0115* MK993574 MK975837 MK975832 龙血树生拟盘多毛孢 P. dracaenicola MFLUCC 18-0913* MN962731 MN962733 MN962732 人面子拟盘多毛孢 P. dracontomelonis MFLU 14-0207* KP781877 NA KP781880 伊顿拟盘多毛孢 P. etonensis BRIP 66615* MK966339 MK977634 MK977635 杜鹃花科拟盘多毛孢 P. ericacearum IFRDCC 2439* KC537807 KC537821 KC537814 台湾拟盘多毛孢 P. formosana NTUCC 17-009* MH809381 MH809385 MH809389 叉状拟盘多毛孢 P. furcata MFLUCC 12-0054* JQ683724 JQ683708 JQ683740 叉状拟盘多毛孢 P. furcata LC6691 KX895030 KX895363 KX895248 白珠树拟盘多毛孢 P. gaultheriae IFRD 411-014 KC537805 KC537819 KC537812 凸圆拟盘多毛孢 P. gibbosa NOF 3175* LC311589 LC311590 LC311591 银桦拟盘多毛孢 P. grevilleae CBS 114127* KM199300 KM199407 KM199504 夏威夷拟盘多毛孢 P. hawaiiensis CBS 114491* KM199339 KM199428 KM199514 西班牙拟盘多毛孢 P. hispanica CBS 115391* MH553981 MH554640 MH554399 荷兰拟盘多毛孢 P. hollandica CBS 265.33* KM199328 KM199388 KM199481 土生拟盘多毛孢 P. humicola CBS 336.97* KM199317 KM199420 KM199484 湖南拟盘多毛孢 P. hunanensis CSUFTCC15* OK493599 OK562374 OK507969 湖南拟盘多毛孢 P. hunanensis CSUFTCC18 OK493600 OK562375 OK507970 弯孢拟盘多毛孢 P. inflexa MFLUCC 12-0270* JX399008 JX399039 JX399072 常孢拟盘多毛孢 P. intermedia MFLUCC 12-0259* JX398993 JX399028 JX399059 意大利拟盘多毛孢 P. italiana MFLU 14-0214* KP781878 KP781882 KP781881 杰斯特拟盘多毛孢 P. jesteri CBS 109350* KM199380 KM199468 KM199554 江西拟盘多毛孢 P. jiangxiensis LC4399* KX895009 KX895341 KX895227 金昌拟盘多毛孢 P. jinchanghensis LC6636* KX895028 KX895361 KX895247 金昌拟盘多毛孢 P. jinchanghensis LC8190 KY464144 KY464164 KY464154 柿拟盘多毛孢 P. kaki KNU-PT-1804* LC552953 LC552954 LC553555 秋茄树生拟盘多毛孢 P. kandelicola NCYU 19-0355* MT560723 MT563100 MT563102 肯尼亚拟盘多毛孢 P. kenyana CBS 442.67* KM199302 KM199395 KM199502 肯尼亚拟盘多毛孢 P. kenyana LC6633 KX895027 KX895360 KX895246 艾氏鱼拟盘多毛孢 P. knightiae CBS 111963 KM199311 KM199406 KM199495 艾氏鱼拟盘多毛孢 P. knightiae CBS 114138* KM199310 KM199408 KM199497 甲米拟盘多毛孢 P. krabiensis MFLUCC 16-0260* MH388360 MH412722 MH388395 木百合拟盘多毛孢 P. leucadendri CBS 121417* MH553987 MH554654 MH554412 轴榈生拟盘多毛孢 P. licualicola HGUP 4057* KC492509 KC481683 KC481684 丽江拟盘多毛孢 P. lijiangensis CFCC 50738* KU860520 KU844184 KU844185 直孢拟盘多毛孢 P. linearis MFLUCC 12-0271* JX398992 JX399027 JX399058 庐山拟盘多毛孢 P. lushanensis LC4344* KX895005 KX895337 KX895223 庐山拟盘多毛孢 P. lushanensis LC8182 KY464136 KY464156 KY464146 庐山拟盘多毛孢 P. lushanensis LC8183 KY464137 KY464157 KY464147 澳洲坚果拟盘多毛孢 P. macadamiae BRIP 63738b KX186588 KX186680 KX186621 澳洲坚果拟盘多毛孢 P. macadamiae BRIP 63739b KX186587 KX186679 KX186620 澳洲坚果拟盘多毛孢 P. macadamiae BRIP 637441a KX186586 KX186678 KX186619 马来西亚拟盘多毛孢 P. malayana CBS 102220* KM199306 KM199411 KM199482 单毛拟盘多毛孢 P. monochaeta CBS 144.97* KM199327 KM199386 KM199479 单毛拟盘多毛孢 P. monochaeta CBS 440.83 KM199329 KM199387 KM199480 南京拟盘多毛孢 P. nanjingensis CSUFTCC16* OK493602 OK562377 OK507972 南宁拟盘多毛孢 P. nanningensis CSUFTCC10* OK493596 OK562371 OK507966 新木姜子拟盘多毛孢 P. neolitseae NTUCC 17-011* MH809383 MH809387 MH809391 新木姜子拟盘多毛孢 P. neolitseae CFCC 54590 OK339744 OK358520 OK358505 纽荷兰拟盘多毛孢 P. novae-hollandiae CBS 130973* KM199337 KM199425 KM199511 稻拟盘多毛孢 P. oryzae CBS 111522 KM199294 KM199394 KM199493 稻拟盘多毛孢 P. oryzae CBS 171.26 KM199304 KM199397 KM199494 稻拟盘多毛孢 P. oryzae CBS 353.69* KM199299 KM199398 KM199496 白茶拟盘多毛孢 P. pallidotheae MAFF 240993* AB482220 NA NA 露兜树生拟盘多毛孢 P. pandanicola MFLUCC 16-0255* MH388361 MH412723 MH388396 巴布亚拟盘多毛孢 P. papuana CBS 331.96* KM199321 KM199413 KM199491 巴布亚拟盘多毛孢 P. papuana CBS 887.96 KM199318 KM199415 KM199492 小孢拟盘多毛孢 P. parva CBS 265.37 KM199312 KM199404 KM199508 小孢拟盘多毛孢 P. parva CBS 278.35* KM199313 KM199405 KM199509 石楠拟盘多毛孢 P. photiniicola GZCC 16-0028* KY092404 KY047663 KY047662 松拟盘多毛孢 P. pini MEAN 1092 MT374680 MT374705 MT374693 松生拟盘多毛孢 P. pinicola KUMCC 19-0183* MN412636 MN417507 MN417509 葡萄牙拟盘多毛孢 P. portugalica CBS 393.48* KM199335 KM199422 KM199510 红树拟盘多毛孢 P. rhizophorae MFLUCC 17-0416* MK764283 MK764349 MK764327 杜鹃花拟盘多毛孢 P. rhododendri IFRDCC 2399* KC537804 KC537818 KC537811 桃金娘拟盘多毛孢 P. rhodomyrtus LC4458 KX895010 KX895342 KX895228 粉红拟盘多毛孢 P. rosea MFLUCC 12-0258* JX399005 JX399036 JX399069 帚状拟盘多毛孢 P. scoparia CBS 176.25* KM199330 KM199393 KM199478 北美红杉拟盘多毛孢 P. sequoiae MFLUCC 13-0399* KX572339 NA NA 圆头拟盘多毛孢 P. spathulata CBS 356.86* KM199338 KM199423 KM199513 剑毛拟盘多毛孢 P. spathuliappendiculata CBS 144035* MH554172 MH554845 MH554607 蒂罗花拟盘多毛孢 P. telopeae CBS 114137 KM199301 KM199469 KM199559 蒂罗花拟盘多毛孢 P. telopeae CBS 114161* KM199296 KM199403 KM199500 蒂罗花拟盘多毛孢 P. telopeae CBS 113606 KM199295 KM199402 KM199498 陆生拟盘多毛孢 P. terricola CBS 141.69* MH554004 MH554680 MH554438 泰国拟盘多毛孢 P. thailandica MFLUCC 17-1616* MK764285 MK764351 MK764329 棕榈生拟盘多毛孢 P. trachicarpicola OP068* JQ845947 JQ845945 JQ845946 棕榈生拟盘多毛孢 P. trachicarpicola IFRDCC 2403 KC537809 KC537823 KC537816 棕榈生拟盘多毛孢 P. trachicarpicola LC4523 KX895011 KX895344 KX895230 同色拟盘多毛孢 P. unicolor MFLUCC 12-0276* JX398999 JX399030 NA 同色拟盘多毛孢 P. unicolor MFLUCC 12-0275 JX398998 JX399029 JX399063 疣孢拟盘多毛孢 P. verruculosa MFLUCC 12-0274* JX398996 NA JX399061 杨凌拟盘多毛孢 P. yanglingensis LC4553* KX895012 KX895345 KX895231 杨凌拟盘多毛孢 P. yanglingensis LC3412 KX894980 KX895312 KX895197 云南拟盘多毛孢 P. yunnanensis HMAS 96359* AY373375 NA NA 注：*表示模式菌株，NA表示无序列。Ex-type strains were marked with * and NA means not available.
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表 3 七种拟盘多毛孢的形态学比较
Table 3. Comparison of 7 Pestalotiopsis species based on conidial morphology.
物种
Species分生孢子长
Conidial
Length/μm分生孢子宽
Conidial
Width/μm长宽比
Length/
width底附属丝长
Length of basal
appendage/μm顶附属丝长
Length of apical
appendage/μm冷杉拟盘多毛孢
P. abietis20.8±0.25C 8.9±0.16A 2.4±0.05D 4.2±0.23C 19.1±0.48AB 矮棕拟盘多毛孢
P. chamaeropis25.8±0.41A 8.0±0.11BC 3.2±0.07B 6.3±0.37B 17.8±0.63B 肯尼亚拟盘多毛孢
P. kenyana25.7±0.57A 7.3±0.09DE 3.6±0.10A 5.6±0.27B 12.6±0.38D 庐山拟盘多毛孢
P. lushanensis23.0±0.32B 8.4±0.12B 2.8±0.05C 7.5±0.24A 18.5±0.54B 南京拟盘多毛孢
P. nanjingensis20.8±0.16C 7.6±0.12CD 2.8±0.05C 4.3±0.16C 14.5±0.35C 葡萄牙拟盘多毛孢
P. portugalica25.4±0.33A 7.7±0.15CD 3.3±0.08AB 4.1±0.26C 20.8±0.52A 桃金娘拟盘多毛孢
P. rhodomyrtus23.0±0.31B 7.0±0.09E 3.3±0.06AB 4.3±0.24C 12.1±0.40D 注：表中数值表示均值±标准误。不同字母表示同一指标在不同物种之间差异显著（P＜0.05）。Values in the table indicate mean ± SE (standard error). Different uppercase letters indicate the same index is different among different species at P＜0.05.
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[1] 陈文艳, 姜宁, 范鑫磊, 等, 2020. 林木病原真菌——赤黏壳科(Erythrogloeaceae)的识别与鉴定[J]. 中国森林病虫, 39(2): 20-27. doi: 10.19688/j.cnki.issn1671-0886.20190033 [2] 姜宁, 2021. 中国栗生子囊菌的分类学研究[D]. 北京: 北京林业大学. [3] Carbone I, Kohn L M, 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes[J]. Mycologia, 91(3): 553-556. doi: 10.1080/00275514.1999.12061051 [4] Chaiwan N, Wanasinghe D N, Mapook A, et al, 2020. Novel species of Pestalotiopsis fungi on Dracaena from Thailand[J]. Mycology, 11(4): 306-315. doi: 10.1080/21501203.2020.1801873 [5] Das K, Lee S Y, Jung H Y, 2021. Pestalotiopsis kaki sp. nov., a novel species isolated from persimmon tree (Diospyros kaki) bark in Korea[J]. Mycobiology, 49(1): 54-60. doi: 10.1080/12298093.2020.1852703 [6] Fan X L, Bezerra J D P, Tian C M, et al, 2020. Cytospora (Diaporthales) in China[J]. Persoonia: Molecular Phylogeny and Evolution of Fungi, 45(1): 1-45. doi: 10.3767/persoonia.2020.45.01 [7] Glass N L, Donaldson, G C, 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes[J]. Applied and Environmental Microbiology, 61(4): 1323-1330. doi: 10.1128/aem.61.4.1323-1330.1995 [8] Gu M, Hu D W, Han B, et al, 2021. Pestalotiopsis abietis sp. nov. from Abies fargesii in China[J]. Phytotaxa, 509(1): 93-105. doi: 10.11646/phytotaxa.509.1.4 [9] Guindon S, Dufayard J F, Lefort V, et al, 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0[J]. Systematic Biology, 59(3): 307-321. doi: 10.1093/sysbio/syq010 [10] Horikawa T, 1986. Yield loss of new tea shoots due to grey blight caused by Pestalotia longiseta Spegazzini[J]. Bulletin of the Shizuoka Tea Experimental Station, 12: 1-8. [11] Hyde K D, Jeewon R, Chen Y J, et al, 2020. The numbers of fungi: is the descriptive curve flattening? [J]. Fungal Diversity, 103(1): 219-271. doi: 10.1007/s13225-020-00458-2 [12] Jeewon R, Liew E C Y, Simpson J A, et al, 2003. Phylogenetic significance of morphological characters in the taxonomy of Pestalotiopsis species[J]. Molecular Phylogenetics and Evolution, 27(3): 372-383. doi: 10.1016/S1055-7903(03)00010-1 [13] Jeewon R, Liew E C Y, Hyde K D, 2004. Phylogenetic evaluation of species nomenclature of Pestalotiopsis in relation to host association[J]. Fungal Diversity, 17: 39-55. [14] Jiang N, Bonthond G, Fan X L, et al, 2018. Neopestalotiopsis rosicola sp. nov. causing stem canker ofRosa chinensis in China[J]. Mycotaxon, 133(2): 271-283. doi: 10.5248/133.271 [15] Jiang N, Fan X L, Tian C M, 2021. Identification and characterization of leaf-inhabiting fungi from Castanea plantations in China[J]. Journal of Fungi, 7(1): 64. doi: 10.3390/jof7010064 [16] Joshi S D, Sanjay R, Baby U I, et al, 2009. Molecular characterization of Pestalotiopsis spp. associated with tea (Camellia sinensis) in southern India using RAPD and ISSR markers[J]. Indian Journal of Biotechnology, 8(4): 377-383. [17] Lee S, Crous P W, Wingfield M J, 2006. Pestalotioid fungi from Restionaceae in the Cape Floral Kingdom[J]. Studies in Mycology, 55(1): 175-187. doi: 10.3114/sim.55.1.175 [18] Li L L, Yang Q, Li H, 2021. Morphology, phylogeny, and pathogenicity of pestalotioid species on Camellia oleifera in China[J]. Journal of Fungi, 7(12): 1080. doi: 10.3390/jof7121080 [19] Liu A R, Chen S C, Wu S Y, et al, 2010. Cultural studies coupled with DNA based sequence analyses and its implication on pigmentation as a phylogenetic marker in Pestalotiopsis taxonomy[J]. Molecular Phylogenetics and Evolution, 57(2): 528-535. doi: 10.1016/j.ympev.2010.07.017 [20] Liu F, Hou L W, Raza M, et al, 2017. Pestalotiopsis and allied genera from Camellia, with description of 11 new species from China[J]. Scientific Reports, 7: 886. doi: 10.1038/s41598-017-00972-5 [21] Liu F, Bonthond G, Groenewald J Z, et al, 2019. Sporocadaceae, a family of coelomycetous fungi with appendage-bearing conidia[J]. Studies in Mycology, 92: 287-415. doi: 10.1016/j.simyco.2018.11.001 [22] Maharachchikumbura S S N, Guo L D, Chukeatirote E, et al, 2011. Pestalotiopsis: morphology, phylogeny, biochemistry and diversity[J]. Fungal Diversity, 50(1): 167-187. doi: 10.1007/s13225-011-0125-x [23] Maharachchikumbura S S N, Guo L D, Cai L, et al, 2012. A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species[J]. Fungal Diversity, 56(1): 95-129. doi: 10.1007/s13225-012-0198-1 [24] Maharachchikumbura S S N, Chukeatirote E, Guo L D, et al, 2013. Pestalotiopsis species associated with Camellia sinensis (tea)[J]. Mycotaxon, 123(1): 47-61. doi: 10.5248/123.47 [25] Maharachchikumbura S S N, Hyde K D, Groenewald J Z, et al, 2014. Pestalotiopsis revisited[J]. Studies in Mycology, 79(1): 121-186. doi: 10.1016/j.simyco.2014.09.005 [26] Norphanphoun C, Jayawardena R S, Chen Y, et al, 2019. Morphological and phylogenetic characterization of novel pestalotioid species associated with mangroves in Thailand[J]. Mycosphere, 10(1): 531-578. doi: 10.5943/mycosphere/10/1/9 [27] O'Donnell K, Cigelnik E, 1997. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous[J]. Molecular Phylogenetics and Evolution, 7(1): 103-116. doi: 10.1006/mpev.1996.0376 [28] Steyaert R L, 1949. Contribution à l'étude monographique de Pestalotia de Not. et Monochaetia Sacc. (Truncatella gen. nov. et Pestalotiopsis gen. nov.)[J]. Bulletin du Jardin Botanique de l'État, Bruxelles, 19(3): 285-347. doi: 10.2307/3666710 [29] Song Y, Geng K, Zhang B, et al, 2013. Two new species of Pestalotiopsis from Southern China[J]. Phytotaxa, 126: 22-30. doi: 10.11646/phytotaxa.126.1.2 [30] White T J, Bruns T, Lee S, et al, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[J]. PCR Protocols: A Guide to Methods and Applications, 18: 315-322. doi: 10.1016/b978-0-12-372180-8.50042-1 [31] Zhu H Y, Pan M, Wijayawardene N N, et al, 2021. The hidden diversity of diatrypaceous fungi in China[J]. Frontiers in Microbiology, 12: 646262. doi: 10.3389/fmicb.2021.646262 -
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